Human BMP-7 / OP-1 (glycosylated, CHO) recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: Osteogenic Protein 1, BMP-7.

Source: CHO

Purity: ≥ 97 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1200.954.002 2 µg 60 €
1200.954.010 10 µg 159 €
1200.954.199 1 mg on request €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Osteogenic Protein 1, BMP-7.

 

Description of Human BMP-7 / OP-1 (glycosylated, CHO)

N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40kDa in SDS-PAGE under non-reducing conditions. BMP-7 is purified by proprietary chromatographic techniques.

 

Biological Activity

Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 70ng/ml, corresponding to a Specific Activity of 14,286IU/mg.

 

Reconstitution

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 0.5mg/1ml sterile/ endotoxin free water.

 

Stability

Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

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Shipping

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