Human BMP-6 (glycosylated) recombinant Protein

Synonyms

Bone morphogenetic protein 6, BMP-6, VG-1-related protein, VG-1-R, VGR-1, BMP6, VGR, VGR1.

Description of Human BMP-6 (glycosylated)

BMP-6 Human Recombinant produced in HEK cells is a glycosylated disulfide linked homodimer of two 139 amino acid polypeptide chains; each monomer is expressed as the C-terminal part of a precursor polypeptide, which contains a 20 amino acid signal peptide and a 354 amino acid propeptide.The BMP-6 which has a total molecular weight of 26.2kDa is purified by proprietary chromatographic techniques.

Biological Activity

The ED₅₀ as determined by its ability to induce alkaline phosphatase production in the ATDC-5 cell line is typically 0.03-0.06 μg/ml.

Reconstitution

It is recommended to reconstitute the lyophilized BMP-6 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized BMP-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

Amino Acid Sequence

VSSASDYNSS ELKTACRKHE LYVSFQDLGW QDWIIAPKGY AANYCDGECS FPLNAHMNAT NHAIVQTLVH LMNPEYVPKP CCAPTKLNAI SVLYFDDNSN VILKKYRNMV VRACGCH