Human IL-3 (glycosylated) recombinant Protein

Synonyms

MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

Description of Human IL-3 (glycosylated)

Interleukin-3 Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 17-45kDa due to glycosylation.The IL-3 is purified by proprietary chromatographic techniques.

Biological Activity

The activity was determined by the dose dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-2ng/ml corresponding to a specific activity of 500,000-2,000,000units/mg.

Reconstitution

It is recommended to reconstitute the lyophilized IL-3 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized IL-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.