Bovine Aprotinin/ Pancreatic Trypsin Inhibitor

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Synonyms

Pancreatic trypsin inhibitor, Basic protease inhibitor, BPI, BPTI, Aprotinin, AP.

Description of Bovine Aprotinin/ Pancreatic Trypsin Inhibitor

Aprotinin is a natural proteinase inhibitor polypeptide consisting of 58 amino acids with a molecular mass of 6,5 kDa It is a single polypeptide chain which is cross-linked by three disulfide bridges. Aprotinin inhibits the activity of several proteolytic enzymes such as chymotrypsin, kallikrein, plasmin and trypsin. Aprotinin is present in blood and most tissues, with a high concentration in lung. Aprotinin inhibits pro-inflammatory cytokine release and maintains glycoprotein homeostasis. In platelets, aprotinin reduces glycoprotein loss (e.g. GpIb, GpIIb/IIIa), while in granulocytes it prevents the expression of pro-inflammatory adhesive glycoproteins (e.g. CD11b). It can be used for in vitro inhibition of fibrinolytic activity in blood samples.

Definition of the units

1 Ph. Eur.-unit of Aprotinin inhibits 50 % of the enzymatic activity of 2 Microkatal Trypsin, with BAEE as Substrate, measured at pH 8,0 und 25 °C.
1 PEU = 1800 KIU.

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of at least 100 µg/ml, which can the be further diluted. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).

Stability

The lyophilized protein is stable at room temperature for up to 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted Aprotinin should be stored in working aliquots at -20°C to -80°C if possible with carrier protein. Please avoid repeated freeze-thaw cycles.

Shipped at room temperature

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