Chicken Growth Hormone recombinant Protein

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Synonyms

GH1, GH, GHN, GH-N, hGH-N,Pituitary GH, GH 1, .

Description of Chicken Growth Hormone

GH Chicken Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids with an additional Ala at its N-terminus and having a molecular mass of 22255 Dalton.

Biological Activity

GH Chicken Recombinantis fully biologically active in homologous assays and in PDF-P1 3B9 cells stably transfected with rabbit GH receptors.

Reconstitution

It is recommended to reconstitute the lyophilized GH Chicken Recombinant in sterile water or 0.4% NaHCO₃ adjusted to pH 8-9, not less than 100µg/ml and not more than 3 mg/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Stability

Lyophilized GH Chicken although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution at > 0.1 mg/ml and filter sterilization GH can be stored at 4°C for several weeks. At lower concentration addition of a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA) is recommended. Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala.

Shipped at room temperature

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