Human GM-CSF (glycosylated, HEK) recombinant Protein

Synonyms

CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897.

Description of Human GM-CSF (glycosylated, HEK)

GM-CSF Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 15-36kDa due to glycosylation.The GM-CSF is purified by proprietary chromatographic techniques.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.4-2ng/ml.

Reconstitution

It is recommended to reconstitute the lyophilized GM-CSF in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized GM-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GM-CSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.