Human VEG-I recombinant Protein

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Synonyms

Tumor necrosis factor ligand superfamily member 15, TNFSF-15, TNFSF15, TNF ligand-related molecule 1, VEGI, TL-1, TL1, TL1A, VEGI192A, VEGI-192, MGC129934, MGC129935.

Description of Human VEG-I

TNFSF15 Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 180 amino acids and having a molecular mass of 20.5kDa.

Biological Activity

The ED₅₀ as determined by its ability to induce apoptosis using human TF-1 cells is less than 20ng/ml, corresponding to a specific activity of > 5.0×104 IU/mg.

Reconstitution

It is recommended to reconstitute the lyophilized TNFSF15 in sterile 18MO-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Stability

TNFSF15 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGI should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

MQLTKGRLHF SHPLSHTKHI SPFVTDAPLR ADGDKPRAHL TVVRQTPTQH FKNQFPALHW EHELGLAFTK NRMNYTNKFL LIPESGDYFI YSQVTFRGMT SECSEIRQAG RPNKPDSITV VITKVTDSYP EPTQLLMGTK SVCEVGSNWF QPIYLGAMFS LQEGDKLMVN VSDISLVDYT KEDKTFFGAF LL

Shipped at room temperature

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