Pufferfish Leptin recombinant Protein

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Synonyms

OB Protein, Obesity Protein, OBS, Obesity factor.

Description of Pufferfish Leptin

Leptin Pufferfish (Takifugu rubripes) Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 16 kDa. Bioactive Leptin Pufferfish (Takifugu rubripes) Recombinant was prepared according to the sequence published by Kurokawa et al. (2005)Peptides 26, 745-750 in two forms: monomer and covalent dimer. MS analysis revealed molecular masses of 15,291 and 30,585 Da, close to the theoretical values of 15,270 and 30,540 Da. CD spectra revealed high similarity to mammalian leptins. Other details of its preparation will be soon published by Yacobovitz et al (in press), General and Comparative Endocrinology.

Biological Activity

Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.

Reconstitution

It is recommended to reconstitute the lyophilized Pufferfish Leptin in sterile 0.4% NaHCO₃ pH-9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability

Lyophilized Pufferfish Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

ALPGALDAMD VEKMKSKVTW KAQGLVARID KHFPDRGLRF DTDKVE GST SVVASLESYN NLISDRFGGV SQIKTEISSL AGYLNHWREG NCQE QQPKV WPRRNIFNHT VSLEALMRVR EFLKLLQKNV DLLERC

Shipped at room temperature

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