E. coli Methionin Aminopeptidase Enzyme

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	Enzymes Synonyms: Methionine aminopeptidase, MAP, Peptidase M, map, b0168, JW0163. Source: E. coli Purity: ≥ 90 % (SDS-PAGE) Stabilizer: 10% glycerol, DTT (2mM) Buffer: Tris-HCl buffer (20mM); pH 8.0 Physical State: Sterile filtered clear solution (1mg/ml) Manufacturer:  Active Bioscience	Article No. Size Price Shopping List 4074.900.005 5 µg 90 € Add 4074.900.020 20 µg 175 € Add 4074.900.199 1 mg 2.390 € Add Please inquire for other vial sizes and custom vialing.

 

 

Produktdetails

Synonyms

Methionine aminopeptidase, MAP, Peptidase M, map, b0168, JW0163.

 

Description of E. coli Methionin Aminopeptidase

Recombinant Methionin Aminopeptidase (MAP) derived from E. coli is a single, non-glycosylated enzyme consisting of 284 amino acids (1-264 a.a.) with a molecular mass of 31.5kDa. Methionin Aminopeptidase is fused to a 20 amino acid His-tag at N-terminus. Methionine Aminopeptidases and designated peptidase M proteins belong to the M24 family of proteins. Methionin Aminopeptidase (MAP) removes the amino-terminal methionine residue from nascent polypeptides. The active site of Methionin Aminopeptidase (MAP) contains 2 adjacent divalent metal ions connected by a water molecule or hydroxide ion.

 

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz). Please avoid repeated freeze-thaw cycles.

 

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Amino Acid Sequence

MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE

 

Shipped with ice packs

 

 

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Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

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