Human MMP-3 (glycosylated, HEK) Enzyme

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Product Overview, Sizes and Prices

 

Enzymes

Synonyms: Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.

Source: HEK

Purity: ≥ 95% (SDS-PAGE)

Physical state: Liquid

Manufacturer:  Active Bioscience

Article No. Size Price  
2145.955.002 2 µg 60 €
2145.955.010 10 µg 159 €
2145.955.199 1 mg 3.990 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Stromelysin-1, EC 3.4.24.17, Matrix metalloproteinase-3, MMP-3, Transin-1, SL-1, STMY, STR1, STMY1, MGC126102, MGC126103, MGC126104.

 

Description of Human MMP-3 (glycosylated, HEK)

MMP-3 Human Recombinant produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus, having an Mw of 52kDa.

 

Biological Activity

The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is > 150 pmoles/min/µg.Recombinant Human MMP-3 protein pro form needs to be activated with Chymotrypsin.Activation Protocol:1. Dilute MMP3 to 20µg/ml in the Assay Buffer: 50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% (w/v) and Brij 35, pH 7.5.2. Activate MMP3 by adding Chymotrypsin(Sigma, Catalog#C­3142,1mg/ml stock in 1mM HCl) to a final concentration of 5ug/ml.3. Incubate at 37°C for 30 minutes.4. Stop activation with 2mM PMSF. Pre-warm the PMSF to 37°C prior to adding to sample.

 

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid repeated freeze-thaw cycles.

 

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Errors, changes and availability excepted.

 

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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