Anti-Human PDGF receptor alpha (MAB) Monoclonal Antibody

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Synonyms

PDGFRA, CD140A, PDGFR2, PDGFR-2, RHEPDGFRA

Description of Anti-Human PDGF receptor alpha (MAB)

PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulfidelinked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF Ra binds all three PDGF isoforms with high affinity, PDGF Rß binds PDG-FBB and AB, but not PDGFAA. Both PDGF Ra and PDGF Rß are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for MCSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo- and heterodimerization and signal transduction. The expression of the a and ß receptors is independently regulated in various cell types. Only PDGF Ra is expressed in oligodendrocyte progenitor cells, mesothelial cell and liver endothelial cells. Soluble PDGFRa has been detected in cell conditioned medium and human plasma. Recombinant soluble PDGF Ra binds PDGF with high affinity and is a potent PDGF antagonist.

Biological Activity

WB: 1:400-1000; IHC: 1:50-500 

Reconstitution

Reconstitute the antibody with 500 µl sterile PBS and the final concentration is 200 µg/ml.

Shipped at room temperature