Bovine FGF-acidic / FGF-1
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Phone: +49 (0) 40 . 43 20 84 48 0
E-Mail: info@active-bioscience.de
Product Overview, Sizes and Prices |
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Protein Synonyms: HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a. Source: Bovine Brain Purity: ≥ 90 %. Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.
Description of Bovine FGF-acidic / FGF-1
Fibroblast Growth Factor-acidic Bovine (FGF-1) purified from Bovine Brain contains a 17 kDa and a 20 kDa polypeptide chain. The 17 kDa peptide is derived from the 20K peptide by restricted proteolysis. (See Jaye et al˛). The FGF acidic is purified by proprietary chromatographic techniques.
Biological Activity
Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF.
Reconstitution
It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Stability
Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18°C. Upon reconstitution aFGF should be stored at 4°C between 2-3 weeks and for future use below -18°C. Please avoid repeated freeze-thaw cycles.
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