Gildhead Seabream IGF-I recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Somatomedin C, IGF-I, IGFI.

Source: E. coli

Purity: ≥ 98 % (SDS-PAGE, SEC-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1480.930.010 10 µg 60 €
1480.930.050 50 µg 159 €
1480.930.199 1 mg 1.890 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Somatomedin C, IGF-I, IGFI.

 

Description of Gildhead Seabream IGF-I

IGF1 Gilthead SeabreamRecombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 7545.4 Dalton, the predicted pI=7.72.IGF-1 is purified by proprietary chromatographic techniques.

 

Biological Activity

Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active.

 

Reconstitution

It is recommended to reconstitute the lyophilized IGF-1 in sterile 0.4% NaHCO3 adjusted to ph 8-9, not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability

Lyophilized IGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF1 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

MSPETLCGAE LVDTLQFVCG ERGFYFSKPG YGPNARRSRG IVDECCFQSC ELRRLEMYCA PAKTSK

 

 

 

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Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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