Human BMP-2 recombinant Protein
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Phone: +49 (0) 40 . 43 20 84 48 0
E-Mail: info@active-bioscience.de
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Protein Synonyms: Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6 Source: E. coli Purity: ≥ 95 % (SDS-PAGE, silver stained) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6
Description of Human BMP-2
Human Bone Morphogenetic Protein-2 (BMP-2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26 kDa. BMP-2 regulates similarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP-2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions. The amino acid sequence of recombinant human BMP-2 starts with MQAKHKQ (position 283) containing the Met from the E. coli expression vector. BMP-2 is a heparin binding protein.
Biological Activity
Measured by the ability of BMP-2 to induce alkaline phosphatase production by C2C12 myogenic cells. The ED50 for this effect is typically 0.3-0.8 µg/ml.
Reconstitution
The lyophilized BMP-2 is best soluble in 50 mM acetic acid at a concentration of 0.1mg/ml but should be also soluble in most aqueous buffers when the pH is below 6.0.
Amino Acid Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
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