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Human BMP-2 recombinant Protein
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Protein Synonyms: Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6. Source: E. coli Biological activity: ≤ 0.8µg/ml Purity: ≥ 95 % (SDS-PAGE, silver stained) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6.
Description of Human BMP-2
Recombinant human BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains. BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
Biological Activity
The ED50 of 0.3-0.8µg/ml was determined by its ability to induce alkaline phosphatase production by C2C12 myogenic cells. It corresponds to a specific activity of ≥ 0.125 U/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in 50mM acetic acid to a concentration of 0.1 mg/ml. It is also be soluble in most aqueous buffers when the pH is below 6.0. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest possible concentration.
Stability
The lyophilized protein is stable at room temperature for up 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted BMP-2 is stable for at least 12 months when stored in working aliquots at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Shipped at room temperature
To buy Human BMP-2 (W1195950005, W1195950010, W1195950050, W1195950100, W1195950250, W1195950500, W1195950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human BMP-2 is an alternative for the no longer available Promokine BMP-2, human, recombinant (E. coli) (C-67310).
All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.
Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
