Human BMP-2 recombinant Protein
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Bone morphogenetic protein 2, BDA2, BMP2A, bone morphogenetic protein 4, ZYME, BMP2B, OFC11, BMP2B1, MCOPS6
Description of Human BMP-2
Recombinant human BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains. BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
The ED50 of 0.3-0.8µg/ml was determined by its ability to induce alkaline phosphatase production by C2C12 myogenic cells. It corresponds to a specific activity of ≥ 0.125 U/mg.
We recommend a quick spin followed by reconstitution in 50mM acetic acid to a concentration of 0.1 mg/ml. It is also be soluble in most aqueous buffers when the pH is below 6.0. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest possible concentration.
The lyophilized protein is stable at room temperature for up 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted BMP-2 is stable for at least 12 months when stored in working aliquots at -20°C to -80°C. Please avoid repeated freeze thaw cycles.
Amino Acid Sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
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