Human BMP-2 (glycosylated, HEK) recombinant Protein
Description of Human BMP-2 (glycosylated, HEK)
Recombinant human BMP-2 glycosylated from HEK is a 28.0 kDa homodimeric protein consisting of two 113 amino acid polypeptide chains. BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.
The ED50 of 5-7ng/ml was determined by its ability to induce alkaline phosphatase production by mouse chondrogenic cell line, ATDC-5.
We recommend a quick spin followed by reconstitution in 4mM HCl containing about 0.1% HSA (highly pure and endotoxin free, 2835.958.xyz). Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest possible concentration.
The lyophilized protein is stable at room temperature for up 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted glycosylated BMP-2 is stable for at least 6 months when stored in working aliquots at -20°C to -80°C with HSA. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR
Shipped at room temperature
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