Human BMP-2 (glycosylated, HEK) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: BMP-2, BMP2A.

Source: HEK

Biological activity: see below

Purity: ≥ 95 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1195.955.002 2 g 65 €
1195.955.010 10 g 165 €
1195.955.100 100 g 1.220 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

BMP-2, BMP2A.

 

Description of Human BMP-2 (glycosylated, HEK)

Recombinant human BMP-2 glycosylated from HEK is a 28.0 kDa homodimeric protein consisting of two 113 amino acid polypeptide chains. BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease.

 

Biological Activity

The ED50 of 5-7ng/ml was determined by its ability to induce alkaline phosphatase production by mouse chondrogenic cell line, ATDC-5.

 

Reconstitution

We recommend a quick spin followed by reconstitution in 4mM HCl containing about 0.1% HSA (highly pure and endotoxin free). Do not vortex. This solution can be stored at 2-8C for up to 1 week or in working aliquots at -20C to -80C. Working aliquots should be at the highest possible concentration.

 

Stability

The lyophilized protein is stable at room temperature for up 1 month and at least until the lot specific expiry date if kept below -18C. Reconstituted glycosylated BMP-2 is stable for at least 6 months when stored in working aliquots at -20C to -80C with HSA. Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

QAKHKQRKRL KSSCKRHPLY VDFSDVGWND WIVAPPGYHA FYCHGECPFP LADHLNSTNH AIVQTLVNSV NSKIPKACCV PTELSAISML YLDENEKVVL KNYQDMVVEG CGCR

 

 

 

 

 

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

 

To buy Human BMP-2 (glycosylated, HEK) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500  within Germany

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