Human BMP-4 (glycosylated, CHO) recombinant Protein
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BMP4, ZYME, BMP2B, BMP2B1.
Description of Human BMP-4 (glycosylated, CHO)
Bone Morphogenetic protein-4 Active Human Recombinant produced in CHO cells is a glycosylated homodimer chain containing 2x116 amino acids and having a total molecular mass of 26.2kDa. BMP4 is purified by proprietary chromatographic techniques.
The ED50, as calculated by Alkaline phosphatase activity induced in ATDC-5 cells is less than 15ng/ml corresponding to a specific activity which is > 6.7 x 104 units/mg.
It is recommended to reconstitute the lyophilized BMP4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized BMP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP4 should be stored at 4°C between 2-7 days and for future use below -18°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
SPKHHSQRAR KKNKNCRRHS LYVDFSDVGW NDWIVAPPGY QAFYCHGDCP FPLADHLNST NHAIVQTLVN SVNSSIPKAC CVPTELSAIS MLYLDEYDKV VLKNYQEMVV EGCGCR
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