Human BMP-7 / OP-1 (glycosylated, HEK) recombinant Protein
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Osteogenic Protein 1, BMP-7.
Description of Human BMP-7 / OP-1 (glycosylated, HEK)
BMP-7 Human Recombinant produced in HEK cells is a glycosylated disulfide-linked homodimer, having a molecular weight range of 30-38kDa due to glycosylation.The BMP7 corresponds to amino acid residues 315 to 431 of the full-length BMP-7 precursor and is purified by proprietary chromatographic techniques.
The specific activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 50-250ng/ml.
It is recommended to reconstitute the lyophilized BMP-7 in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Lyophilized BMP7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-7 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
DFSLDNEVHS SFIHRRLRSQ ERREMQREIL SILGLPHRPR PHLQGKHNSA PMFMLDLYNA MAVEEGGGPG GQGFSYPYKA VFSTQGPPLA SLQDSHFLTD ADMVMSFVNL VEHDKEFFHP RYHHREFRFD LSKIPEGEAV TAAEFRIYKD YIRERFDNET FRISVYQVLQ EHLGRESDLF LDSRTLWASE EGWLVFDITA TSNHWVVNPR HNLGLQLSVE TLDGQSINPK LAGLIGRHGP QNKQPFMVAF FKAT
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