Human Cardiotrophin-1, CTF1 recombinant Protein
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Protein Synonyms: CTF1, CT1, CT-1, Cardiophin 1, Cardiotrophin-1. Source: E. coli Biological activity: ≥ 1 x 106 IU/mg Purity: ≥ 95 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
CTF1, CT1, CT-1, Cardiophin 1, Cardiotrophin-1.
Description of Human Cardiotrophin-1, CTF1
Cardiotrophin-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 201 amino acids and having a molecular mass of 21.2kDa.The CTF1 is purified by proprietary chromatographic techniques.
Biological Activity
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg.
Reconstitution
It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
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