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Human Cathepsin L
Native whole molecule & biologically active
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Proteins Synonyms: CatL, Major excreted protein (MEP), CTSL Biological Activity: > 1 unit/mg of protein Source: Human Liver Purity: ≥ 95% by SDS-PAGE Physical State: Frozen in 20 mM Malonate, pH 5.5, 1 mM EDTA, 400 mM NaCl. Manufacturer: |
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Athens Research & Technology
Produktdetails
Synonyms
CatL, Major excreted protein (MEP), CTSL
Description of Human Cathepsin L
Human Cathepsin L is a 25000 - 29000 Da protein. Cathepsin L (CTSL) is one of the most potent lysosomal cysteine endopeptidases, demonstrating significantly higher specific activity than cathepsins B and H in degrading physiological protein substrates. As a member of the papain-like protease family, CTSL plays crucial roles in protein catabolism, antigen processing, bone resorption, and ovulation. CTSL is widely expressed in eukaryotic cells, functioning primarily within lysosomes but also secreted extracellularly. It efficiently degrades various substrates including extracellular matrix proteins (laminins, fibronectin, collagens), cytoplasmic, and nuclear proteins. CTSL exhibits specificity for aromatic residues in the P2 position with limited prime-site selectivity4. CTSL is significantly upregulated in multiple cancer types, particularly breast cancer, where it serves as a powerful predictor of relapse and survival following treatment. Its ability to degrade extracellular matrix facilitates tumor invasion and metastasis. CTSL plays key roles in, coronary heart disease progression SARS-CoV-2 infection, and pulmonary emphysema development. CTSL represents a promising therapeutic target, with inhibitors showing potential in preventing cancer metastasis, treating COVID-19 by blocking viral entry mechanisms, and managing atherosclerosis progression
Source
Prepared from liver shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Biological Activity
> 1 unit/mg of protein. One unit is defined as the amount of enzyme that hydrolyzes 1 µmol of Z-Phe-Arg-AFC per minute at 25 °C using 400 mM sodium acetate, pH 5.5 with 4 mM EDTA, and 8 mM DTT as the activation buffer in the presence of Brij.
Storage
For long term, store Human Cathepsin L at ≤ -80°C.
Applications
Cancer, SARS-CoV-2, Emphysema, Atherosclerosis, In Vitro Diagnostic.
Citations/Publications
Lambeth, T. R., et al., (2021), 'Proteolysis of Amyloid β by Lysosomal Enzymes as a Function of Fibril Morphology', ACS Omega, 6: pp 31520−31527. Available at:https://doi.org/10.1021/acsomega.1c03915
Sanman, L. E., et al., (2016), 'Bifunctional Probes of Cathepsin Protease Activity and pH Reveal Alterations in Endolysosomal pH during Bacterial Infection', Cell Chemical Biology, 23(7): pp 793 - 804. Available at: http://dx.doi.org/10.1016/j.chembiol.2016.05.019
Mellott, D. M., et al., (2021), 'A Clinical-Stage Cysteine Protease Inhibitor blocks SARS-CoV‑2 Infection of Human and Monkey Cells', ACS Chem. Biol., 16: pp 642−650. Available at: https://doi.org/10.1021/acschembio.0c00875
NCBI: https://www.ncbi.nlm.nih.gov/protein/P07711
Shipped with dry ice
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
Paying by bank transfer
