Human CD14, soluble (glycosylated) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

Source: CHO

Purity: ≥ 95 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1239.954.010 10 µg 60 €
1239.954.050 50 µg 159 €
1239.954.199 1 mg on request €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14.

 

Description of Human CD14, soluble (glycosylated)

The CD14 is produced from human CD14 transfected CHO-cells. Before transfection the complete human CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wild type CD14 is the 50-kDa protein containing 335 amino acids and lacks the GPI-Anchor.

 

Biological Activity

Up to 20 µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 600,000 CD14+CHO transfectants (FACS).

 

Reconstitution

It is recommended to reconstitute the lyophilized CD14 in sterile 18MO-cm H2O not less than 100µg/ml. Further dilutions should be made with phosphate buffered saline (PBS).

 

Stability

Lyophilized CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD14 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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