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Human Chylomicrons
Native whole molecule
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Proteins Synonyms: CHY Source: Human Plasma Purity: Single arc by IEP against antisera to whole human serum. Essentially free of other plasma lipoproteins as determined by electrophoresis using a SPIFE Vis Cholesterol gel kit for lipids and Coomassie Blue for proteins. ≥ 95% of total lipoprotein content by electrophoresis. Physical State: Liquid in 150 mM NaCl, pH 7.4, and 0.01% EDTA Manufacturer: |
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Athens Research & Technology
Produktdetails
Synonyms
CHY
Description of Human Chylomicrons
Human Chylomicrons is a 50000-1000000 Da protein. Chylomicrons are enterocyte-derived triglyceride-rich lipoproteins that transport dietary lipids from the intestine to peripheral tissues and the liver. These particles (~75–1, 200 nm diameter) consist of a hydrophobic core of triglycerides (85–92%) and cholesterol esters, surrounded by a phospholipid monolayer containing apolipoproteins. ApoB-48, synthesized exclusively in the intestine through mRNA editing of the APOB gene, serves as the structural scaffold for chylomicron assembly. During circulation, chylomicrons acquire apoE and apoC-II from HDL, with the latter activating lipoprotein lipase (LPL) to hydrolyze triglycerides into free fatty acids for tissue uptake. Post-lipolysis remnants, enriched in cholesterol and apoE, are cleared by hepatic receptors (LDL-R, LRP1). Familial chylomicronemia syndrome caused by LPL deficiency or mutations in cofactors (apoC-II, GPIHBP1), leading to severe hypertriglyceridemia (>2, 000 mg/dL) and pancreatitis risk. Chylomicron retention disease (CRD), an autosomal recessive SAR1B mutations impair chylomicron secretion, causing vitamin E deficiency, neuropathy, and growth failure. Postprandial chylomicron remnants contribute to atherosclerosis via endothelial dysfunction and proinflammatory pathways. Quantifying apoB-48 provides a specific biomarker for intestinal lipoprotein metabolism, aiding cardiovascular risk stratification.
Source
Prepared from fresh, non-frozen plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Storage
For long term, store Human Chylomicrons at ≤ -20°C.
Applications
Lipid Metabolism, Cardiovascular Disease.
Citations/Publications
Moreno-Gordaliza, E., et al., (2016), 'A novel method for serum lipoprotein profiling using high performance capillary isotachophoresis', Analytica Chimica Acta 944: pp 57-69. Available at: http://dx.doi.org/10.1016/j.aca.2016.09.038
Nimonkar, A. V., et al., (2019), 'A lipoprotein lipase–GPI-anchored high-density lipoprotein–binding protein 1 fusion lowers triglycerides in mice: Implications for managing familial chylomicronemia syndrome', J. Biol. Chem., 295(10): pp 2900–2912. Available at: DOI 10.1074/jbc.RA119.011079
Frontera, L. S., et al., (2018), 'Lactoferrin and lactoferricin endocytosis halt Giardia cell growth and prevent infective cyst production', Scientific Reports., 8: pp 18020. Available at: DOI:10.1038/s41598-018-36563-1
Shipped with ice packs
Gel Scan of Human Chylomicrons
| Fig. 1: SDS-PAGE Gel |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
Paying by bank transfer
