Human Collagen Type IV alpha 3 recombinant Protein
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Collagen alpha-3(IV) chain, Goodpasture antigen, COL4A3, Glomerular Basal Membrane, GBM.
Description of Human Collagen Type IV alpha 3
Human α3 chain of collagen IV; identical with the antigen called "glomerular basal membrane antigen" (GBM). Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Calculated Molecular weight: 53kDa. Calculated isoelectric point: pH 8.9. cDNA coding for a minicollagen version of the human collagen IV α3 chain fused to a hexa-histidine purification tag. The term minicollagen designates the removal of most of the epitope-less triplehelical collagenous region (situated between the N-terminal 7S domain and the C-terminal noncollagenous NC1 domain), which is a requirement for recombinant production of this antigen.
Recommendations for storage buffer: neutral to slightly alkaline pH and 4M urea as dissociating agent. Storage temperature: -70°C to -80°C. Please avoid repeated freeze-thaw cycles.
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