Human EGF recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Epidermal growth factor, EGF, URG, HOMG4, Urogastrone.

Source: E. coli

Biological activity: ≥ 2.0 x 105 units/mg

Purity: ≥ 95 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1325.950.100 100 µg 60 €
1325.950.200 200 µg 90 €
1325.950.500 500 µg 150 €
1325.950.199 1 mg 180 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Epidermal growth factor, EGF, URG, HOMG4, Urogastrone.

 

Description of Human EGF

Recombinant human Epidermal Growth Factor (EGF) is a 6.35 kDa globular protein containing 54 amino acid residues including 3 intramolecular disulfide-bonds. EGF is a potent growth factor, which stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals trough a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation and colony formation of epidermal cells in culture.

 

Biological Activity

The ED50 of ≤ 5 ng/ml was determined by its ability to stimulate the proliferation of primary NHDF cells. It corresponds to a specific activity of ≥ 2.0 x 105 units/mg. This corresponds to an activity of ≥ 1.0 x 107 units/mg in a mouse BALB/c 3T3 assay.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of at least 100µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer - if possible - containing a carrier (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

 

Stability

The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and below -20°C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots with a carrier at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.

 

Lupe Zoom

Amino Acid Sequence

MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

 

Shipping symbol Shipped at room temperature

 

 

 

 

 

 

 

 

To buy Human EGF (W1325950100, W1325950200, W1325950500, W1325950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human EGF is an alternative for the no longer available Promokine EGF, human, recombinant (E. coli) (C-60170).

 

All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.

 

Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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