Human EGF (Yeast) recombinant Protein
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Protein Synonyms: Urogastrone, URG, EGF. Source: Yeast, Pichia pastoris Purity: ≥ 98 % (SDS-PAGE, RP-HPLC) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Urogastrone, URG, EGF.
Description of Human EGF (Yeast)
Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa.The EGF is purified by proprietary chromatographic techniques.
Biological Activity
The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 107 Units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.
Stability
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW E
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Customer Service
Phone: +49 (0) 40 . 43 20 84 48 0
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Payment Methods
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