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Human Elastase
Native whole molecule, biologically active
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Proteins Synonyms: Neutrophil Elastase, HNE, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE, ELANE, ELA2 Biological Activity: 20-22 units per mg protein Source: Human Neutrophils Purity: ≥ 95% by SDS-PAGE Physical State: Salt-free lyophilized solid. Manufacturer: |
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Athens Research & Technology
Produktdetails
Synonyms
Neutrophil Elastase, HNE, Bone marrow serine protease, Elastase-2, Human leukocyte elastase, Medullasin, PMN elastase, HLE, ELANE, ELA2
Description of Human Elastase
Human Elastase is a 29.5 kDa protein. Neutrophil elastase (NE), a serine protease stored in azurophilic granules of neutrophils, plays critical roles in host defense and tissue remodeling. As a member of the chymotrypsin superfamily, NE hydrolyzes elastin, collagen, and proteoglycans through its catalytic triad (His-Asp-Ser), enabling pathogen clearance via microbial membrane disruption. Beyond direct bactericidal activity, NE modulates immune responses by activating matrix metalloproteinases (MMPs), inducing pro-inflammatory cytokines (TNF-α, IL-1β, IL-8), and promoting neutrophil extracellular trap (NET) formation. However, unchecked NE activity drives extracellular matrix degradation, contributing to chronic inflammatory and degenerative diseases. NE dysregulation is central to pulmonary emphysema, particularly in α1-antitrypsin deficiency, where insufficient protease inhibition leads to alveolar destruction. In chronic obstructive pulmonary disease (COPD), NE excess exacerbates airway inflammation and mucus hypersecretion, perpetuating lung parenchymal damage. NE also directly injures cartilage in rheumatoid arthritis by degrading collagen and activating fibroblast-like synoviocytes, amplifying joint inflammation. Additionally, elevated NE levels correlate with cystic fibrosis progression, acute respiratory distress syndrome (ARDS), and severe COVID-19, where it enhances viral entry and cytokine storms. Balancing NE’s protective and pathogenic roles remains a clinical challenge, necessitating therapies that preserve antimicrobial function while preventing tissue destruction.
Source
Neutrophils shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
Biological Activity
20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of MeO-Suc-Ala-Ala-Pro-Val-pNA per minute at 25oC, pH 7.5.
Storage
For long term, store Human Elastase at ≤ -20°C.
Applications
Host Defense, Antimicrobial, Inflammation, Cancer, NETosis, Emphysema, COPD, SARS-CoV-2, Rheumatoid Arthritis, In Vitro Diagnostic.
Citations/Publications
Hiroyasu, S., et al., (2021), 'Granzyme B inhibition reduces disease severity in autoimmune blistering diseases', NATURE COMMUNICATIONS, 12: pp 302. Available at: https://doi.org/10.1038/s41467-020-20604-3
Nielsen, N. S., et al., (2022), 'Cryo-EM structures of human A2ML1 elucidate the protease-inhibitory mechanism of the A2M family', NATURE COMMUNICATIONS., 13: pp 3033. Available at: https://doi.org/10.1038/s41467-022-30758-x
Li, Y., et al., (2024), 'Cystine-knot peptide inhibitors of HTRA1 bind to a cryptic pocket within the active site region', Nature Communications 15: pp 4359. Available at: https://doi.org/10.1038/s41467-024-48655-w
Lee, C. W., et al., (2017) 'The lysosomal protein cathepsin L is a progranulin protease', Molecular Neurodegeneration, 12: pp 55. Available at: DOI 10.1186/s13024-017-0196-6
Alatrash, G., et al., (2018), ' Targeting the Leukemia Antigen PR1 with Immunotherapy for the Treatment of Multiple Myeloma', Clin Cancer Res., 24(14): pp 3386-3396. Available at: doi: 10.1158/1078-0432.CCR-17-2626.
NCBI: https://www.ncbi.nlm.nih.gov/protein/P08246
Shipped with ice packs
Gel Scan of Human Elastase
| Fig. 1: SDS-PAGE Gel |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
Paying by bank transfer
