Human Erythropoietin, EPO-a-Fc (glycosylated, CHO) recombinant Protein

Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: EPO-a, EPO-alpha, Epoetin, EP, MGC138142.

Source: CHO

Biological activity: ≥ 5 x 105 IU/mg

Purity: ≥ 98 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1345.954.002 2 µg 60 €
1345.954.010 10 µg 159 €
1345.954.199 1 mg on request €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

EPO-a, EPO-alpha, Epoetin, EP, MGC138142.

 

Description of Human Erythropoietin, EPO-a-Fc (glycosylated, CHO)

Erythropoietin-alpha Fc-Chimera Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a dimeric, glycosilated, polypeptide chain consisting of two mature human EPO molecules linked to the Fc portion of human IgG1. The Fc component contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 140 kDa in non-reducing SDS-PAGE.

 

Biological Activity

The ED50 as determined by the dose-dependent stimulation of human megakaryoblastic leukemia cells is less than 2.0 ng/ml, corresponding to a Specific Activity of 5.0 x 105 IU/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.

 

Stability

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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