Human Erythropoietin, EPO-a (glycosylated, CHO) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Erythropoietin-Alpha, EPO-a, EPO-alpha, EP, MGC138142.

Source: CHO

Purity: ≥ 98 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1346.954.005 5 µg 60 €
1346.954.050 50 µg 159 €
1346.954.199 1 mg 1.150 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Erythropoietin-Alpha, EPO-a, EPO-alpha, EP, MGC138142.

 

Description of Human Erythropoietin, EPO-a (glycosylated, CHO)

Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques.

 

Biological Activity

The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.

 

Reconstitution

It is recommended to reconstitute the lyophilized EPO-alpha in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

Stability

Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR

 

 

 

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