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Human Fetuin-A/AHSG (glycosylated, HEK) recombinant Protein
Human Fetuin A, alpha-2-HS-glycoprotein, glycosylated; HEK
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Proteins Synonyms: Alpha-2-HS-glycoprotein, Fetuin-A, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, AHSG, FETUA, AHS, A2HS, HSGA, PRO2743. Source: HEK Biological Activity: see below Purity: ≥ 98 % (SDS-PAGE, RP-HPLC) Physical State: Lyophilized Manufacturer: |
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Active Bioscience
Produktdetails
Synonyms
Alpha-2-HS-glycoprotein, Fetuin-A, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, AHSG, FETUA, AHS, A2HS, HSGA, PRO2743.
Description of Human Fetuin-A/AHSG (glycosylated, HEK)
Recombinant human Fetuin A produced in HEK cells is a glycosylated heterodimeric protein of 309 amino acids (A-chain with 282 aa; B-chain with 27 aa). It has a molecular mass of 32,9 kDa, but due to glycosylation migrates at 45-55 kDa in SDS-Page under non-reducing conditions. Fetuin A is a human plasma glycoprotein belonging to the Cystatin family of protease inhibitors. It is highly expres¬sed in embryonic cells and adult hepatocytes and is expressed to a lesser extent in monocytes/ma¬cro¬phages. Fetuin A is a major serum protein component that exerts various calcium-dependent physiological activities and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-ß and BMP signaling proteins.
Biological Activity
The IC50 of ≤ 100nM was determined by its ability to inhibit Cathespin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (RLUs).
Reconstitution
We recommend a quick spin followed by reconstitution in sterile Water at a concentration of 0.1 mg/ml, which can then be further diluted into other aqueous or buffered solutions.
Stability
The lyophilized protein is stable for at least 1 year from date of receipt if stored at -20°C to -80°C. Reconstituted Fetuin A should be stored at 4°C if entire vial will be used within 1-2 weeks. It is stable for least 3 months when stored in working aliquots with a carrier protein at -20°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
A-Chain:
APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL
B-Chain:
TVVQPSVG AAAGPVVPPC PGRIRHFKV
Shipped at room temperature
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
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