Human Fetuin-A/AHSG (glycosylated, HEK) recombinant Protein
Alpha-2-HS-glycoprotein, Fetuin-A, Alpha-2-Z-globulin, Ba-alpha-2-glycoprotein, AHSG, FETUA, AHS, A2HS, HSGA, PRO2743.
Description of Human Fetuin-A/AHSG (glycosylated, HEK)
Recombinant human Fetuin A produced in HEK cells is a glycosylated heterodimeric protein of 309 amino acids (A-chain with 282 aa; B-chain with 27 aa). It has a molecular mass of 32,9 kDa, but due to glycosylation migrates at 45-55 kDa in SDS-Page under non-reducing conditions. Fetuin A is a human plasma glycoprotein belonging to the Cystatin family of protease inhibitors. It is highly expres¬sed in embryonic cells and adult hepatocytes and is expressed to a lesser extent in monocytes/ma¬cro¬phages. Fetuin A is a major serum protein component that exerts various calcium-dependent physiological activities and can mediate growth signaling in certain tumor cells. It also can act as a natural antagonist against specific TGF-ß and BMP signaling proteins.
The IC50 of ≤ 100nM was determined by its ability to inhibit Cathespin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (RLUs).
We recommend a quick spin followed by reconstitution in sterile Water at a concentration of 0.1 mg/ml, which can then be further diluted into other aqueous or buffered solutions.
The lyophilized protein is stable for at least 1 year from date of receipt if stored at -20°C to -80°C. Reconstituted Fetuin A should be stored at 4°C if entire vial will be used within 1-2 weeks. It is stable for least 3 months when stored in working aliquots with a carrier protein at -20°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
APHGPGLIYR QPNCDDPETE EAALVAIDYI NQNLPWGYKH TLNQIDEVKV WPQQPSGELF EIEIDTLETT CHVLDPTPVA RCSVRQLKEH AVEGDCDFQL LKLDGKFSVV YAKCDSSPDS AEDVRKVCQD CPLLAPLNDT RVVHAAKAAL AAFNAQNNGS NFQLEEISRA QLVPLPPSTY VEFTVSGTDC VAKEATEAAK CNLLAEKQYG FCKATLSEKL GGAEVAVTCT VFQTQPVTSQ PQPEGANEAV PTPVVDPDAP PSPPLGAPGL PPAGSPPDSH VL
TVVQPSVG AAAGPVVPPC PGRIRHFKV
Shipped at room temperature
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