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Human FGF-10 / KGF-2, cct-premium recombinant Protein
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Protein Synonyms: FGF10, KGF2, KGF-2, FGFA. Source: E. coli Biological activity: ≥ 2 x 106 units/mg Purity: ≥ 95 % (SDS-PAGE, silver stained) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
FGF10, KGF2, KGF-2, FGFA.
Description of Human FGF-10 / KGF-2, cct-premium
Recombinant Human Fibroblast Growth Factor-10 (FGF-10 or KGF-2) is a 170 amino acid protein, with a molecular mass of 19,3 kDa. It is a heparin-binding growth factor belonging to the fibroblast growth factor (FGF) family most related to KGF/FGF-7. FGF family members possess extensive mitogenic and cell survival functions, and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-10 is expressed during development and preferentially in adult lungs. It signals through FGFR 2b.
Biological Activity
Assay 1: The ED50 of ≤ 0.5ng/ml was determined by the dose-dependent stimulation of thymidine uptake of BaF3 cells expressing FGFRs. It corresponds to a specific activity of ≥ 2.0 x 106 units/mg.
Assay 2: An ED50 of 0,5-1,0 ng/ml was determined by the FGF-10 induced proliferation of 4MBr-5 cells.
Reconstitution
We recommend a quick spin followed by reconstitution in water to a concentration of at least 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week or in working aliquots at -20°C to -80°C. Working aliquots should be at the highest practical concentration. For long term storage we recommend to add at least 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA.
Stability
The lyophilized protein is stable at room temperature for up to 1 month and at least until the lot specific expiry date if kept below -18°C. Reconstituted FGF-10 should be stored in working aliquots at -20°C to -80°C if possible with carrier protein. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS
Shipped at room temperature
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Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
