Human FGF-11, Isoform 1 recombinant Protein
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FGF 11 Iso 1, FGF11 Iso1.
Description of Human FGF-11, Isoform 1
Recombinant Human Fibroblast Growth Factor-11 Isoform 1 (FGF-11 Iso1) is a 225 amino acid protein, with C-terminal His-Tag with a molecular mass of 22,5 kDa without His-Tag. It belongs to the fibroblast growth factor (FGF) family. FGF family members possess extensive mitogenic and cell survival functions, and are involved in various biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The expression pattern of the FGF-11 mouse homolog implies a role in nervous system development.
The ED50 of ≤ 0.2 ng/ml was determined by the dose-dependent proliferation of NIH/3T3 cells. It corresponds to a specific activity of ≥ 5.0 x 106 units/mg.
We recommend a quick spin followed by reconstitution in water to a concentration of at least 100 µg/ml. Do not vortex. Please allow sufficient time, at least 20 min, for complete reconstitution. This solution can then be further diluted into working aliquots and stored at -20°C to -80°C. Working aliquots should be at the highest practical concentration and preferably be used within one month after reconstitution.
The lyophilized protein is stable at room temperature for up to 2 weeks and at least until the lot specific expiry date if kept below -18°C. Reconstituted FGF-11 should be stored in suitable working aliquots at -20°C to -80°C and used within one month after reconstitution. Please avoid repeated freeze thaw cycles.
Amino Acid Sequence
MAALASSLIR QKREVREPGG SRPVSAQRRV CPRGTKSLCQ KQLLILLSKV RLCGGRPARP DRGPEPQLKG IVTKLFCRQG FYLQANPDGS IQGTPEDTSS FTHFNLIPVG LRVVTIQSAK LGHYMAMNAE GLLYSSPHFT AECRFKECVF ENYYVLYASA LYRQRRSGRA WYLGLDKEGQ VMKGNRVKKT KAAAHFLPKL LEVAMYQEPS LHSVPEASPS SPPAPHHHHH H
Shipped at room temperature
Fig. 1: SDS-PAGE analysis of recombinant human FGF-11 Isoform 1
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