Human FGF-basic, FGF-2 recombinant Protein

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Product Overview, Sizes and Prices



Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2

Source: E. coli

Biological activity: ≥ 5 x 105 IU/mg (HUVEC); ≥ 1 x 107 units/mg (BALB/c 3T3)

Purity: ≥ 98 % (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1369.950.050 50 g 140 €
1369.950.200 200 g 170 €
1369.950.250 250 g 200 €
1369.950.500 500 g 360 €
1369.950.199 1 mg 590 €

Please inquire for other vial sizes and custom vialing.



Product Details


FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2


Description of Human FGF-basic, FGF-2

Recombinant human Fibroblast Growth Factor-basic (hFGF-b) is a 16.5 kDa protein containing 153 amino acid residues. Interestingly, hFGF-b contains no hydrophobic leader sequence previously thought to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues. hFGF-b is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. It also exerts a potent angiogenic activity in vivo. Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. In general FGFs induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. One of the most potentially significant applications of FGF-b is related to its reported ability to induce angiogenesis.


Biological Activity

The ED50 of ≤ 2ng/ml was determined for stimulation of cell proliferation with human FGF-basic (FGF-2) in HUVECs (human umbilical vein endothelial cells), corresponding to a specific activity of ≥ 5x105 IU/mg. An ED50 of ≤ 0.1 ng/ml was determined by stimulation of 3H-Thymidine incorporation in BALB/c 3T3 cells, corresponding to a specific activity of ≥ 1.0 x 107 units/mg. Maximal effect for DNA synthesis was observed at 1.0 -2.0 ng/ml recombinant human FGF-basic.



We recommend a quick spin followed by reconstitution in water to a concentration at least 0.05 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA or HSA) and store in working aliquots at -20C to -80C for up to 12 months.



The lyophilized protein is stable below -20C until expiry date. Reconstituted human FGF-basic should be stored in working aliquots at -20C. Please avoid repeated freeze-thaw cycles.


Amino Acid Sequence





Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.



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