Human FGF-basic, FGF-2 recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2

Source: E. coli

Purity: ≥ 98 % (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1369.950.050 50 µg 140 € €
1369.950.200 200 µg 170 € €
1369.950.250 250 µg 200 € €
1369.950.500 500 µg 360 € €
1369.950.199 1 mg 590 € €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2

 

Description of Human FGF-basic, FGF-2

FGF basic (FGF2, HBGF2) is one of at least 23 mitogenic proteins of the FGF family, which show 35-60% amino acid conservation. Unlike other FGFs, FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans (HSPG) are likely. The predicted 17 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted. Transcription from alternate start sites produces 21-24 kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression of different genes when expressed in NIH3T3 cells. The 17 kDa mouse sequence has 98% aa identity with rat, and 95% identity with human, bovine and sheep FGF basic. Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity. Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological conditions.

 

Biological Activity

The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by human FGF-2 (basic) has been determined to be in the range of 0.1-2 ng/ml.

 

Reconstitution

The lyophilized FGF-2 (basic) should be reconstituted in water to a concentration not lower than 50 µg/ml. For long term storage we would recommend to add at least 0.1% human or bovine serum albumin.

 

Amino Acid Sequence

AGSITTLPAL PEDGGSGAFP PGHFKDPKRL YCKNGGFFLR IHPDGRVDGV REKSDPHIKL QLQAEERGVV SIKGVCANRY LAMKEDGRLL ASKCVTDECF FFERLESNNY NTYRSRKYTS WYVALKRTGQ YKLGSKTGPG QKAILFLPMS AKS

 

 

 

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