Human FGF-basic, FGF-2 (glycosylated, HEK) recombinant Protein
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Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Description of Human FGF-basic, FGF-2 (glycosylated, HEK)
FGF-2 Human Recombinant Thermostable produced in HEK cells is a non-glycosylated monomer, containing 154 amino acids and having a total molecular weight of 17kDa.FGF-2 Thermostable is a protein engineered FGF2 in order to enhance its thermostability without modifying its biological function.The FGF-b is purified by proprietary chromatographic techniques.
The specific activity was determined by the dose dependent stimulation of proliferation of the Balb/c 3T3 cell line, the ED50 is 0.03ng/ml.
It is recommended to reconstitute the lyophilized FGF-b in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Lyophilized FGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
PALPEDGGSG AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH IKLQLQAEER GV VSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYK
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