EN
Human FGF-basic-thermostable, FGF-2-thermostable recombinant Protein
Product Overview, Sizes and Prices |
||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
|
Protein Synonyms: FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2. Source: E. coli Biological activity: ≥ 1 x 106 IU/mg (HUVEC); ≥ 2 x 107 units/mg (BALB/c 3T3) Purity: ≥ 98 % (SDS-PAGE, silver stained) Physical state: Lyophilized Manufacturer: |
|
||||||||||||||||||||||||
Active Bioscience
Product Details
Synonyms
FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2.
Description of Human FGF-basic-thermostable, FGF-2-thermostable
Recombinant human Fibroblast Growth Factor-basic (hFGF-b) thermostable is a 16.5 kDa protein containing 153 amino acid residues. It has been generated by a targeted exchange of 8 amino acids in comparison with the wild type. FGF-Basic is an essential factor in stem cell culture. Recombinant FGF-Basic has a very long shelf life if it is stored lyophilized or dissolved at low temperatures but degrades quickly in cell culture medium at higher tempera-tures. The thermostability of FGF-2 in medium can be increased by specific mutations. Our FGF-2-thermostable is characterized by an increased thermostability compared to the wild type while maintaining the same high activity (Fig. 1).
Biological Activity
The ED50 of ≤ 1 ng/ml was determined for stimulation of cell proliferation with human FGF-basic (FGF-2) in HUVECs (human umbilical vein endothelial cells), corresponding to a specific activity of ≥ 1x106 IU/mg. An ED50 of ≤ 0.05 ng/ml was determined by stimulation of 3H-Thymidine incorporation in BALB/c 3T3 cells, corresponding to a specific activity of ≥ 2.0 x 107 units/mg.
Reconstitution
We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration of at least 0.05 mg/ml. Do not vortex. This solution can then be diluted into other aqueous buffers. For extended storage, it is rec-ommended to further dilute in a buffer containing a carrier protein e.g. 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).
Stability
The lyophilized protein is stable at room temperature for up to 3 weeks and below -20°C until expiry date. Reconstitut-ed human FGF-basic thermostable should be stored in working aliquots at -20°C. Please avoid repeated freeze-thaw cycles.
Shipped at room temperature
Stability of Thermostable FGF-basic
| Fig. 1: Stability of Thermostable FGF-basic after 8 Days of Incubation - Proliferation with HUVEC (HU2007-02). |
To buy Human FGF-basic-thermostable, FGF-2-thermostable please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.
All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.
Errors, changes and availability excepted.
Payment Methods
Paying by bank transfer
