Human FGF-basic-thermostable, FGF-2-thermostable recombinant Protein
FGF2, BFGF, FGFB, HBGF-2, basic Fibroblast growth factor (bFGF), Heparin binding growth factor-2.
Description of Human FGF-basic-thermostable, FGF-2-thermostable
Recombinant human Fibroblast Growth Factor-basic (hFGF-b) thermostable is a 16.5 kDa protein containing 153 amino acid residues. It has been generated by a targeted exchange of 8 amino acids in comparison with the wild type. FGF-Basic is an essential factor in stem cell culture. Recombinant FGF-Basic has a very long shelf life if it is stored lyophilized or dissolved at low temperatures but degrades quickly in cell culture medium at higher tempera-tures. The thermostability of FGF-2 in medium can be increased by specific mutations. Our FGF-2-thermostable is characterized by an increased thermostability compared to the wild type while maintaining the same high activity (Fig. 1).
The ED50 of ≤ 1 ng/ml was determined for stimulation of cell proliferation with human FGF-basic (FGF-2) in HUVECs (human umbilical vein endothelial cells), corresponding to a specific activity of ≥ 1x106 IU/mg. An ED50 of ≤ 0.05 ng/ml was determined by stimulation of 3H-Thymidine incorporation in BALB/c 3T3 cells, corresponding to a specific activity of ≥ 2.0 x 107 units/mg.
We recommend a quick spin followed by reconstitution in sterile-filtered water to a concentration of at least 0.05 mg/ml. Do not vortex. This solution can then be diluted into other aqueous buffers. For extended storage, it is rec-ommended to further dilute in a buffer containing a carrier protein e.g. 0.1% HSA (order number: 2835.955.xyz or 2835.958.xyz) or BSA (order number: 2835.919.xyz).
The lyophilized protein is stable at room temperature for up to 3 weeks and below -20°C until expiry date. Reconstitut-ed human FGF-basic thermostable should be stored in working aliquots at -20°C. Please avoid repeated freeze-thaw cycles.
Shipped at room temperature
Stability of Thermostable FGF-basic
Fig. 1: Stability of Thermostable FGF-basic after 8 Days of Incubation - Proliferation with HUVEC (HU2007-02).
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