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Description of Human Fibronectin
Human Fibronectin produced purified from Human Plasma having a Molecular Weight of 450kDa.
Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 hours. The recommended concentration is 4M-5M urea. When using the protein as an attachment factor, wash the urea off after attaching the fibronectin to the growth surface (plate or dish).
Store the lyophilized fibronectin at 4°C. Upon reconstitution fibronectin should be stored at 4°C for 2 weeks and for future use below -18°C. Please avoid repeated freeze-thaw cycles.
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