Human Flt-1 / VEGFR-1 soluble (D4) (InCs) recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: soluble vascular endothelial growth factor receptor-1, soluble FLT1, soluble VEGFR-1

Source: Insect cells

Purity: ≥ 90 % (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1397.952.005 5 µg 130 €
1397.952.020 20 µg 250 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

soluble vascular endothelial growth factor receptor-1, soluble FLT1, soluble VEGFR-1

 

Description of Human Flt-1 / VEGFR-1 soluble (D4) (InCs)

Recombinant Human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-4 (sVEGFR-1(D4)) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 4 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 55 kDa containing 457 amino acid residues. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular split tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVEC supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.

 

Biological Activity

The activity of sVEGFR-1(D4) was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.

 

Reconstitution

The lyophilized sVEGFR-1(D4) is soluble in water and most aqueous buffers and should be reconstituted in PBS to a concentration not lower than 100µg/ml.

 

Amino Acid Sequence

SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHRKQQVLET VAGKRSYRLS MKVKAFPSPE VVWLKDGLPA TEKSARYLTR GYSLIIKDVT EEDAGNYTIL LSIKQSNVFK NLTATLIVNV KPQIYEKAVS SFPDPALYPL GSRQILTCTA YGIPQPTIKW FWHPCNHNHS EARCDFC

 

 

 

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