Human Flt3-Ligand (glycosylated, HEK) recombinant Protein
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Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Description of Human Flt3-Ligand (glycosylated, HEK)
Flt3-Ligand Human Recombinant produced in HEK293 cells is a glycosylated, polypeptide chain, migrates as a diffuse band on SDS-PAGE due to heterogeneous glycosylation at 30kDa. Flt3-Ligand is purified by proprietary chromatographic techniques.
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml (Specific Activity: 2.0 x 1,000,000 – 1.0 x 1,000,000 units/mg).
We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP
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