Human GM-CSF recombinant Protein

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Synonyms

CSF-2, MGI-1GM, Pluripoietin-alpha.

Description of Human GM-CSF

Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14.5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific α chain and a common β chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Biological Activity

The ED₅₀ of ≤ 0.1ng/ml was determined by the dose dependent proliferation of TF-1 cells. It corresponds to a specific activity of ≥ 1 x 10⁶ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile Water at a concentration of 0.1 mg/ml, which can then be further diluted into other aqueous or buffered solutions.

Stability

The lyophilized protein is stable for at least 1 year from date of receipt if stored at -20°C to -80°C. Reconstituted hGM-CSF can be stored at 4°C if entire vial will be used within 1-2 weeks or in working aliquots at -20°C. We recommend the addition of a carrier protein (e.g. HSA, order number: 2835.958.xyz) for long sterm storage and especially for high dilutions. Please avoid repeated freeze-thaw cycles.

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Amino Acid Sequence

APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Shipped at room temperature

Purity and Biological Activity

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