Human GM-CSF, cct-premium recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: CSF2, GMCSF.

Source: E. coli

Biological activity: ≥ 1 x 107 IU/mg

Purity: ≥ 98 % (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1450.950.025 25 g 175 €
1450.950.050 50 g 270 €
1450.950.100 100 g 490 €
1450.950.200 200 g 850 €
1450.950.500 500 g 1.490 €
1450.950.199 1 mg 2.350 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

CSF2, GMCSF.

 

Description of Human GM-CSF, cct-premium

Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific a chain and a common chain that is shared by the high-affinity receptors for IL-3 and IL-5.

 

Biological Activity

The ED50 of ≤ 0.1 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. This corresponds to a specific activity of ≥ 1.0 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1 mg/ml. Do not vortex. This solution can then be diluted into other aqueous buffers and stored at 4C for up to 1 week or at -20C for future use.

 

Stability

The lyophilized protein is stable at room temperature for up to 3 weeks and below -20C until expiry date. Reconstituted human GM-CSF should be stored in working aliquots at -20C. Please avoid repeated freeze-thaw cycles.

 

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Amino Acid Sequence

APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

 

Shipping symbol Shipped at room temperature

 

 

 

SDS-PAGE and Biological Activity of Human GM-CSF, cct-premium

<strong>Fig. 1</strong>: SDS-PAGE analysis of recombinant Human GM-CSF, cct-premium. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain.

Fig. 1: SDS-PAGE analysis of recombinant Human GM-CSF, cct-premium. Sample was loaded in 15% SDS-polyacrylamide gel under reducing condition and stained with Silver stain.

<strong>Fig. 2</strong>: Dose-dependent stimulation of cell proliferation in TF-1 cells by recombinant Human GM-CSF, cct-premium and the WHO standard 88/646. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

Fig. 2: Dose-dependent stimulation of cell proliferation in TF-1 cells by recombinant Human GM-CSF, cct-premium and the WHO standard 88/646. Values are the means (SD) of triplicate determinations and expressed as percentage of control.

 

 

 

 

 

 

 

 

 

To buy Human GM-CSF, cct-premium (W1450950025, W1450950050, W1450950100, W1450950200, W1450950500, W1450950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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