Human GM-CSF, cct-premium (HSA) recombinant Protein

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Synonyms

CSF2, GMCSF.

Description of Human GM-CSF, cct-premium (HSA)

Recombinant human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), a 14,5 kDa protein consisting of 127 amino acid residues (Ala18-Glu144), is a potent species specific stimulator of bone marrow cells and several other cell types. GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoietic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is species specific and human GM-CSF has no biological effects on mouse cells. GM-CSF exerts its biological effects through binding to specific cell surface receptors. The high affinity receptors required for human GM-CSF signal transduction have been shown to be heterodimers consisting of a GM-CSF-specific a chain and a common ß chain that is shared by the high-affinity receptors for IL-3 and IL-5.

Biological Activity

The ED₅₀ of ≤ 0.1 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. This corresponds to a specific activity of ≥ 1.0 x 10⁷ units/mg.

Reconstitution

We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration of 0.1 mg/ml. Do not vortex. This solution can then be diluted into other aqueous buffers and stored at 4°C for up to 1 week or at -20°C for future use.

Stability

The lyophilized GM-CSF, although stable at room temperature for 3 weeks, is best stored desiccated at -20°C. Reconstituted GM-CSF should be stored in working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA, order number: 2835.955.xyz or 2835.958.xyz, or BSA, order number: 2835.919.xyz).

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Amino Acid Sequence

APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE

Shipped at room temperature

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