Human HGF, cct-premium (glycosylated, InCs) recombinant Protein
HGF, SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Scatter factor, rh HGF.
Description of Human HGF, cct-premium (glycosylated, InCs)
Recombinant human Hepatocyte Growth Factor (HGF) is a 78.0 kDa disulfide-linked heterodimeric protein. HGF is a multifunctional polypeptide that acts in a paracrine fashion on a wide variety of cell types. Human HGF, also know as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. Hepatocytes have to be primed (e.g. TNF and IL-6) before they can fully respond to HGF. Recent studies suggest that HGF synergizes with FGF-basic in the induction of angiogenesis.
The ED50 of ≤ 3 ng/ml was determined by scattering activity in MDCK cells in direct comparison with WHO Standard 96/564. It corresponds to a specific activity of ≥ 3.3x105 units/mg.
The lyophilized HGF, cct-premium is best soluble in 50mM acetic acid. It should be reconstituted to a concentration of 100µg/ml. We recommend that further dilutions should be made into buffer containing carrier protein (e.g. HSA ultra pure, 2835.958.xyz) or medium containing serum.
The lyophilized protein, though stable at room temperature for at least 3 weeks, is best stored desiccated below -18°C. Reconstituted HGF, cct-premium should be stored in working aliquots at -20°C to -70°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR
VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS
Shipped at room temperature
SDS-PAGE and Biological Activity of Human HGF, cct-premium (glycosylated, InCs)
Fig 1: SDS-PAGE analysis of recombinant human HGF, cct-premium derived from insect cells. Sample was loaded in 10% SDS-polyacrylamide gel under non-reducing conditions and stained with silver stain.
Fig 2: Measurement of scattering activity in MDCK cells by recombinant human HGF, cct-premium and the WHO standard 96/564. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
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