Human HGF (glycosylated, HEK) recombinant Protein
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Phone: +49 (0) 40 . 43 20 84 48 0
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Protein Synonyms: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF. Source: HEK Purity: ≥ 90 % (SDS-PAGE) Physical state: Lyophilized Manufacturer: |
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Active Bioscience
Product Details
Synonyms
Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF.
Description of Human HGF (glycosylated, HEK)
HGF Human Recombinant produced in HEK cells is a glycosylated disulfide-linked heterodimer, containing 697 a.a. (Gln-32 to Ser-728) having a total molecular weight of 80kDa. The HGF is purified by proprietary chromatographic techniques.
Biological Activity
The activity was measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells (ATCC CCL-208). The EC50 for this effect is typically 20-40ng/ml.
Reconstitution
It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized HGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
32-QRKRRNT IHEFKKSAKT TLIKIDPALK IKTKKVNTAD QCANRCTRNK GLPFTCKAFV FDKARKQCLW FPFNSMSSGV KKEFGHEFDL YENKDYIRNC IIGKGRSYKG TVSITKSGIK CQPWSSMIPH EHSFLPSSYR GKDLQENYCR NPRGEEGGPW CFTSNPEVRY EVCDIPQCSE VECMTCNGES YRGLMDHTES GKICQRWDHQ TPHRHKFLPE RYPDKGFDDN YCRNPDGQPR PWCYTLDPHT RWEYCAIKTC ADNTMNDTDV PLETTECIQG QGEGYRGTVN TIWNGIPCQR WDSQYPHEHD MTPENFKCKD LRENYCRNPD GSESPWCFTT DPNIRVGYCS QIPNCDMSHG QDCYRGNGKN YMGNLSQTRS GLTCSMWDKN MEDLHRHIFW EPDASKLNEN YCRNPDDDAH GPWCYTGNPL IPWDYCPISR CEGDTTPTIV NLDHPVISCA KTKQLRVVNG IPTRTNIGWM VSLRYRNKHI CGGSLIKESW VLTARQCFPS RDLKDYEAWL GIHDVHGRGD EKCKQVLNVS QLVYGPEGSD LVLMKLARPA VLDDFVSTID LPNYGCTIPE KTSCSVYGWG YTGLINYDGL LRVAHLYIMG NEKCSQHHRG KVTLNESEIC AGAEKIGSGP CEGDYGGPLV CEQHKMRMVL GVIVPGRGCA IPNRPGIFVR VAYYAKWIHK IILTYKVPQS -728
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