Human HGF (glycosylated, InCs) recombinant Protein
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HGF, SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Scatter factor, rh HGF
Description of Human HGF (glycosylated, InCs)
Human Hepatocyte Growth Factor (HGF), also known as scatter factor, is a pleiotropic cytokine that shows homology to the enzymes of the blood coagulation cascade. It stimulates the motility and invasion of several cancer cell types and can induce angiogenesis. Recently HGF was found to be identical to scatter factor, a fibroblast-derived factor promoting the dissociation of epithelial and vascular endothelial cell colonies in monolayer cell cultures by stimulating cell migration. HGF is synthesized as a biologically inactive single chain precursor, which is cleaved by a specific, extracellular serum serine protease to a fully active heterodimer. This mature, biologically active HGF consists of a disulfide-linked alpha-beta heterodimer of the two cleavage products. Previous studies have shown that single chain and heterodimeric HGF are equally active in vitro assay systems due to either production of the serine protease in cell culture or the presence of the ubiquitous protease in serum. All biological responses induced by HGF are elicited by binding to its transmembrane tyrosine kinase receptor, which is encoded by the MET proto-oncogene. After autophosphorylation of the receptor different cytoplasmic effectors are activated that bind to the same multifunctional docking site of the receptor. HGF function is essential for normal development. Knockout studies have demonstrated that both ligand and receptor deficient mice display an embryonic lethal phenotype. Hepatocytes have to be primed before they can fully respond to HGF. This priming requires cytokines as TNF and IL-6. Recent studies have suggested that HGF synergizes with basic FGF in the induction of angiogenesis.
The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 1.0 - 5.0 ng/ml.
The lyophilized human HGF is soluble in acetic acid (50 mM) and can be reconstituted to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
Amino Acid Sequence
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS YRGKDLQENY CRNPRGEEGG PWCFTSNPEV RYEVCDIPQC SEVECMTCNG ESYRGLMDHT ESGKICQRWD HQTPHRHKFL PERYPDKGFD DNYCRNPDGQ PRPWCYTLDP HTRWEYCAIK TCADNTMNDT DVPLETTECI QGQGEGYRGT VNTIWNGIPC QRWDSQYPHE HDMTPENFKC KDLRENYCRN PDGSESPWCF TTDPNIRVGY CSQIPNCDMS HGQDCYRGNG KNYMGNLSQT RSGLTCSMWD KNMEDLHRHI FWEPDASKLN ENYCRNPDDD AHGPWCYTGN PLIPWDYCPI SRCEGDTTPT IVNLDHPVIS CAKTKQLRVV NGIPTRTNIG WMVSLRYRNK HICGGSLIKE SWVLTARQCF PSRDLKDYEA WLGIHDVHGR GDEKCKQVLN VSQLVYGPEG SDLVLMKLAR PAVLDDFVST IDLPNYGCTI PEKTSCSVYG WGYTGLINYD GLLRVAHLYI MGNEKCSQHH RGKVTLNESE ICAGAEKIGS GPCEGDYGGP LVCEQHKMRM VLGVIVPGRG CAIPNRPGIF VRVAYYAKWI HKIILTYKVP QS
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