Human HGF (glycosylated, InCs) recombinant Protein
HGF, SF, HGFB, HPTA, F-TCF, DFNB39, Hepatocyte growth factor, Scatter factor, rh HGF.
Description of Human HGF (glycosylated, InCs)
Recombinant human Hepatocyte Growth Factor (HGF) is a 78.0 kDa disulfide-linked heterodimeric protein. HGF is a multifunctional polypeptide that acts in a paracrine fashion on a wide variety of cell types. Human HGF, also know as scatter factor, is a pleiotrophic cytokine that shows homology to the enzymes of the blood coagulation cascade. Hepatocytes have to be primed (e.g. TNF and IL-6) before they can fully respond to HGF. Recent studies suggest that HGF synergizes with FGF-basic in the induction of angiogenesis.
The ED50 of ≤ 5 ng/ml was determined by scattering activity in MDCK cells. It corresponds to a specific activity of ≥ 2x105 units/mg.
The lyophilized HGF is soluble in acetic acid 50mM. The lyophilized product should be reconstituted to a concentration of 100µg/ml. It is recommended that further dilutions be made into buffer containing carrier protein (e.g. HSA ultra pure, 2835.958.xyz) or medium containing serum.
The lyophilized protein, though stable at room temperature, is best stored desiccated below -18°C. Reconstituted HGF should be stored in working aliquots at -20°C to -70°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR
VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS
Shipped at room temperature
Biological Activity of Human HGF (glycosylated, InCs)
Fig 1: Measurement of scattering activity in MDCK cells by recombinant human HGF and a competitor. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
To buy Human HGF (glycosylated, InCs) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human HGF (glycosylated, InCs) is an alternative for the no longer available Promokine HGF, human, recombinant (Sf9) (C-64530).
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