Human IGF-I LR3 recombinant Protein

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+49 (0) 40 . 43 20 84 48 0

 

Product Overview, Sizes and Prices

 

Protein

Synonyms: Long R3 IGF-I, Somatamedin C, IGF-IA.

Source: E. coli

Biological activity: ≥ 1.0 x 105 units/mg (rat L6); ≥ 5.0 x 105 units/mg (mouse FDC-P1)

Purity: ≥ 98 % (SDS-PAGE, HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1483.950.250 250 g 60 €
1483.950.500 500 g 120 €
1483.950.199 1 mg 159 €
1483.950.599 5 mg 620 €
1483.950.109 10 mg on request
1483.950.509 50 mg on request
1483.950.166 100 mg on request

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Long R3 IGF-I, Somatamedin C, IGF-IA.

 

Description of Human IGF-I LR3

Recombinant Human IGF-I LR3 is a 9.1 kDa, single, nonglycosylated polypeptide chain containing 83 amino acid residues. The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissues in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions. Recombinant human IGF-I and IGF-II are globular proteins containing 70 and 67 amino acids, respectively, and 3 intra-molecular disulfide bonds. IGF-I LR3 is a recombinant analog of human IGF-I comprised of the complete IGF-I sequence, with an Arginine substitution for the third position Glutamic acid, and a 13 amino acid length N terminus peptide extension. Specifically engineered for higher biological potencysin vitro, IGF-I LR3 has an increased half-life and a binding aversion to native proteins within the body that make it ideal for both research and large-scale culturing.

 

Application

Recombinant Human IGF-I LR3 may increase cell growth and productivity in cell culture using CHO, ES, NK, HSC, MSC, fibroblast, hybridomas and many other cell types with a Type I IGF receptor. Typical working concentrations are between 10g/L and 100 g/L. 50g/L is a good starting concentration. Some cells might need sequential adaptation (25/75; 50/50; 75/25; 0/100). Adaptation usually works best if started when cells are in mid-logarithmic growth phase and at ≥ 90% viability.

 

Biological Activity

The ED50 of ≤ 10.0 ng/ml was determined by its ability to stimulate the proliferation of rat-L6 myoblast cell line. It corresponds to a specific activity of ≥ 1.0 x 105 units/mg. In mouse FDC-P1 cells it shows a ED50 of ≤ 2.0 ng/ml corresponding to a specific activity of ≥ 5.0 x 105 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in 100mM acetic acid or water to a concentration of 1.0 mg/ml. Do not vortex. Mix thoroughly to ensure complete dissolving of IGF-I LR3. We recommend keeping working aliquots at the highest practical concentration and if possible to use for example LoBind Eppendorf tubes for the preparation of working aliquots to prevent protein absorption.

 

Stability

The lyophilized protein is stable at room temperature for up to 1 month, and unopened at 2C -8C until expiry date. Reconstituted protein is stable for at least 12 months when stored in working aliquots at 2C-8C or at -18C to -24C. Do not store below -24C. Please avoid repeated freeze-thaw cycles.

 

Lupe Zoom

Amino Acid Sequence

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

 

Shipping symbol Shipped at room temperature

 

 

 

 

 

 

 

 

To buy Human IGF-I LR3 please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable. Human IGF-I LR3 is an alternative for the no longer available Promokine IGF-1 (R3), human, recombinant (C-60839).

 

All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.

 

Errors, changes and availability excepted.

 

 

 

Payment Methods

Paying by bank transfer

Shipping

We ship with UPS

Free shipping for orders above 500 € within Germany

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