Human IGF-II recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.

Source: E. coli

Biological activity: ≥ 1 x 106 units/mg

Purity: ≥ 97 % (SDS-PAGE, RP-HPLC)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1481.950.010 10 g 60 €
1481.950.050 50 g 159 €
1481.950.199 1 mg 1.150 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

Somatomedin-A, IGF2, INSIGF, pp9974, C11orf43, FLJ22066, FLJ44734.

 

Description of Human IGF-II

Recombinant Human IGF-II is a globular protein containing 67 amino acids, It has 3 intra-molecular disulfide bonds and a molecular mass of 7.5 kDa. The IGFs are mitogenic, polypeptide growth factors that stimulate the proliferation and survival of various cell types, including muscle, bone, and cartilage tissue in vitro. IGFs are predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGFs belong to the Insulin gene family, which also contains insulin and relaxin. The IGFs are similar to insulin by structure and function, but have a much higher growth-promoting activity than insulin. IGF-II expression is influenced by placenta lactogen, while IGF-I expression is regulated by growth hormone. Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/Mannose-6-phosphate receptor. Mature IGFs are generated by proteolytic processing of inactive precursor proteins, which contain N-terminal and C-terminal propeptide regions.

 

Biological Activity

The ED50 of ≤ 1ng/ml was determined by the dose dependent stimulation the proliferation of MCF-7 cells. It corresponds to a specific activity of ≥ 1 x 106 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in water to a concentration of at least 0.1 mg/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week or in working aliquots at -20C to -80C. Working aliquots should be at the highest practical concentration. For extended storage, it is recommended to further dilute in a buffer containing a carrier (e.g. 0.1% HSA order number: 2835.955.xyz or 2835.958.xyz) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable at room temperature for up 1 month and at least until the lot specific expiry date if kept below -18C. Reconstituted IGF-II is stable for at least 6 months when stored in working aliquots at -20C to -80C. Please avoid repeated freeze thaw cycles.

 

Amino Acid Sequence

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

 

 

 

 

 

To buy Human IGF-II (W1481950010, W1481950050, W1481950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

Payment Methods

Paying by bank transfer

Shipping

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Free shipping for orders above 500  within Germany

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