Human IL-27 (glycosylated, HEK) recombinant Protein
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Interleukin-30, IL-30, IL-27/p28, p28, Interleukin-27, Interleukin-27/p28, IL-27, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL27-A, Il27, Il27a, IL-27p28.
Description of Human IL-27 (glycosylated, HEK)
IL-27 Human Recombinant produced in HEK cells is a glycosylated non-covalently heterodimer that consists of 2 chains, IL27A (IL27p28) having a Mw of 25kDa and IL27B (EBI3) having a Mw of 30kDa. Each chain migrates separately on SDS-PAGE. The IL-27 protein is purified by proprietary chromatographic techniques
The specific activity was determined by the dose dependent inhibition of HIV replication in human CD4+ T cells and in human monocyte-derived macrophages and is typically 4-12ng/ml.
It is recommended to reconstitute the lyophilized IL-27 in sterile PBS containing 0.1% endotoxin-free recombinant HSA.
Lyophilized IL-27 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL27 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
IL-27A(215 aa):FPRPPG RPQLSLQELR REFTVSLHLA RKLLAEVRGQ AHRFAESHLP GVNLYLLPLG EQLPDVSLTF QAWRRLSDPE RLCFISTTLQ PFHALLGGLG TQGRWTNMER MQLWAMRLDL RDLQRHLRFQ VLAAGFNLPE EEEEEEEEEE EERKGLLPGA LGSALQGPAQ VSWPQLLSTY RLLHSLELVL SRAVRELLLL SKAGHSVWPL GFPTLSPQP. IL-27B(209 aa):RKGPPA ALTLPRVQCR ASRYPIAVDC SWTLPPAPNS TSPVSFIATY RLGMAARGHS WPCLQQTPTS TSCTITDVQL FSMAPYVLNV TAVHPWGSSS SFVPFITEHI IKPDPPEGVR LSPLAERQLQ VQWEPPGSWP FPEIFSLKYW IRYKRQGAAR FHRVGPIEAT SFILRAVRPR ARYYVQVAAQ DLTDYGELSD WSLPATATMS LGK
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