Human IL-3, cct-premium recombinant Protein

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Product Overview, Sizes and Prices

 

Protein

Synonyms: IL3, IL-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

Source: E. coli

Biological activity: ≥ 1 x 107 IU/mg

Purity: ≥ 98% (SDS-PAGE, silver stained)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1509.950.010 10 g 165 €
1509.950.050 50 g 320 €
1509.950.100 100 g 510 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Synonyms

IL3, IL-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor

 

Description of Human IL-3, cct-premium

Recombinant human Interleukin-3 (IL-3) is a 15.0 kDa globular protein containing 134 amino acid residues. It is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.

 

Biological Activity

The ED50 of ≤ 0.1 ng/ml was determined by the dose dependent stimulation of the proliferation of TF-1 cells. It corresponds to a specific activity of ≥ 1 x 107 units/mg.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration not less than 100 g/ml. Do not vortex. This solution can be stored at 2-8C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.

 

Stability

The lyophilized protein is stable at room temperature for up to 1 month, at 4C for up to 6 months, and at -20C to -80C until expiry date. Working aliquots stored with a carrier protein are stable for at least 12 months at -20C to -80C. Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF

 

Shipping symbol Shipped at room temperature

 

 

 

Purity and Biological Activity of recombinant human IL-3, cct-premium (E. coli)

Fig. 1: SDS-PAGE analysis of recombinant human IL-3,cct-premium. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and visualized with Silver staining.

Fig. 1: SDS-PAGE analysis of recombinant human IL-3,cct-premium. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and visualized with Silver staining.

Fig. 2: Proliferation assay with TF1 cells. The cells were stimulated using recombinant human IL-3, cct-premium and the WHO standard 91/510.

Fig. 2: Proliferation assay with TF1 cells. The cells were stimulated using recombinant human IL-3, cct-premium and the WHO standard 91/510.

 

 

 

 

 

 

 

 

Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.

 

To buy Human IL-3, cct-premium please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button Shopping List & Order Form or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.

 

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Errors, changes and availability excepted.

 

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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