Human IL-3, cct-premium recombinant Protein
IL3, IL-3, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor.
Description of Human IL-3, cct-premium
Recombinant human Interleukin-3 (IL-3) is a 15.0 kDa globular protein containing 134 amino acid residues. It is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoieses, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is species-specific, variably glycosylated cytokine.
The ED50 of ≤ 0.1 ng/ml was determined by the dose dependent stimulation of the proliferation of TF-1 cells. It corresponds to a specific activity of ≥ 1 x 107 units/mg.
We recommend a quick spin followed by reconstitution in sterile filtered water to a concentration not less than 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
The lyophilized protein is stable at room temperature for up to 1 month, at 4°C for up to 6 months, and at -20°C to -80°C until expiry date. Working aliquots stored with a carrier protein are stable for at least 12 months at -20°C to -80°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
Shipped at room temperature
Purity and Biological Activity of recombinant human IL-3, cct-premium (E. coli)
Fig. 1: SDS-PAGE analysis of recombinant human IL-3,cct-premium. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions and visualized with Silver staining.
Fig. 2: Proliferation assay with TF1 cells. The cells were stimulated using recombinant human IL-3, cct-premium and the WHO standard 91/510.
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