Human IL-35 (glycosylated, HEK) recombinant Protein

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Phone: +49 (0) 40 . 43 20 84 48 0

E-Mail: info@active-bioscience.de

 

Product Overview, Sizes and Prices

 

Protein

Source: HEK

Purity: ≥ 95 % (SDS-PAGE)

Physical state: Lyophilized

Manufacturer:  Active Bioscience

Article No. Size Price  
1594.955.002 2 µg 60 €
1594.955.010 10 µg 159 €
1594.955.100 100 µg 850 €

Please inquire for other vial sizes and custom vialing.

 

 

Product Details

Description of Human IL-35 (glycosylated, HEK)

IL35 Human Recombinant produced in HEK 293 cells is comprised of a p35 subunit from IL-12 (Accession # p29459) and a EBI3 (Accession # q14213) subunit from IL-27. IL35 is a heterodimeric polypeptide chain containing 442 amino acids, with a predicted molecular weight of 49 kDa. From N terminus to C terminus, the molecule is comprised of a poly His tag, the EBI3 subunit, a G rich linker and the p35 subunit.

 

Reconstitution

We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.

 

Stability

Lyophilized IL35 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL35 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.

 

Amino Acid Sequence

EBI3subuni t:RKGPPAAL TLPRVQCRAS RYPIAVDCSW TLPPAPNSTS PVSFIATYRL GMAARGHSWP CLQQTPTSTS CTITDVQLFS MAPYVLNVTA VHPWGSSSSF VPFITEHIIK PDPPEGVRLS PLAERQLQVQ WEPPGSWPFP EIFSLKYWIR YKRQGAARFH RVGPIEATSF ILRAVRPRAR YYVQVAAQDL TDYGELSDWS LPATATMSLG Kp35subuni t:RNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS

 

 

 

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Errors, changes and availability excepted.

 

 

Payment Methods

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Shipping

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Free shipping for orders above 500 € within Germany

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