Human IL-35 (glycosylated, HEK) recombinant Protein
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Description of Human IL-35 (glycosylated, HEK)
IL35 Human Recombinant produced in HEK 293 cells is comprised of a p35 subunit from IL-12 (Accession # p29459) and a EBI3 (Accession # q14213) subunit from IL-27. IL35 is a heterodimeric polypeptide chain containing 442 amino acids, with a predicted molecular weight of 49 kDa. From N terminus to C terminus, the molecule is comprised of a poly His tag, the EBI3 subunit, a G rich linker and the p35 subunit.
We recommend a quick spin followed by reconstitution in sterile water to a concentration of at least 100 µg/ml, which can then be further diluted to other aqueous solutions. Do not vortex.
Lyophilized IL35 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL35 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
EBI3subuni t:RKGPPAAL TLPRVQCRAS RYPIAVDCSW TLPPAPNSTS PVSFIATYRL GMAARGHSWP CLQQTPTSTS CTITDVQLFS MAPYVLNVTA VHPWGSSSSF VPFITEHIIK PDPPEGVRLS PLAERQLQVQ WEPPGSWPFP EIFSLKYWIR YKRQGAARFH RVGPIEATSF ILRAVRPRAR YYVQVAAQDL TDYGELSDWS LPATATMSLG Kp35subuni t:RNLPVATP DPGMFPCLHH SQNLLRAVSN MLQKARQTLE FYPCTSEEID HEDITKDKTS TVEACLPLEL TKNESCLNSR ETSFITNGSC LASRKTSFMM ALCLSSIYED LKMYQVEFKT MNAKLLMDPK RQIFLDQNML AVIDELMQAL NFNSETVPQK SSLEEPDFYK TKIKLCILLH AFRIRAVTID RVMSYLNAS
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