Human IL-4, cct-premium recombinant Protein
IL4, BCGF, BCDF, B cell stimulating factor (BSF-1).
Description of Human IL-4, cct-premium
Recombinant human Interleukin-4, cct-premium is a 14.9 kDa globular protein containing 130 amino acid residues. IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naïve CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, and IL-13, which favour a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.
The ED50 of ≤ 0.12 ng/ml was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. In direct comparison with the WHO standard (NIBSC 88/656) it corresponds to a specific activity of ≥ 8 x 106 IU/mg.
We recommend a quick spin followed by reconstitution in water to a concentration of at least 0.1mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted IL-4 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. Please avoid repeated freeze-thaw cycles.
Amino Acid Sequence
MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Shipped at room temperature
Purity and Biological Activity of Human IL-4, cct-premium
Fig. 1: SDS-PAGE analysis of recombinant human IL-4, cct-premium. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver staining.
Fig. 2: Proliferation assay with TF-1 cells. The cells were stimulated using recombinant human IL-4, cct-premium and the WHO standard 88/656. The cells were stimulated with increasing amounts of the recombinant proteins. Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
To buy Human IL-4, cct-premium (W1515950005, W1515950025, W1515950050, W1515950100, W1515950200, W1515950500, W1515950199) please click on the Shopping List Symbol right from the indicated price. This will add the product to your Shopping List. You can then click on the button »Shopping List & Order Form« or the Shopping List Symbol in the top navigation to ask for a quote. We will send you your quote as soon as possible. Our offer will include institutional discounts where applicable.
All prices are net prices (excluding VAT). Final prices in our quote will contain VAT plus shipping costs if applicable. For further information please go to our Shipping page.
Errors, changes and availability excepted.
Paying by bank transfer
We ship with UPS
Free shipping for orders above 500 € within Germany