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Human IL-6 recombinant Protein
Human Interleukin-6, recombinant
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Proteins Synonyms: IL6, HGF, HSF, BSF2, IL-6, IFNB2. Source: E. coli Purity: ≥ 98 % (SDS-PAGE, silver stained) Physical State: Lyophilized Manufacturer: |
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Active Bioscience
Produktdetails
Synonyms
IL6, HGF, HSF, BSF2, IL-6, IFNB2.
Description of Human IL-6
Interleukin 6 (IL-6) is a pleiotropic a-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa-28 kDa phosphorylated and variably glycosylated molecule. Mature human IL6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. Human IL6 is equally active on mouse and rat cells. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/ IL-6 R but not from other cytokines that utilize gp130 as a co-receptor.
Biological Activity
The ED50 as determined by the dose-dependent stimulation of murine hybridoma B9 cells is in the range of ≤ 10 - 25 pg/ml.
Reconstitution
The lyophilized IL-6 should be reconstituted in water to a concentration not less than 100µg/ml. This solution can be diluted into other buffered solutions or stored at -20 °C for future use.
Amino Acid Sequence
MAPVPPGEDS KDVAAPHRQP LTSSERIDKQ IRYILDGISA LRKETCNKSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEETCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PDPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSSL RALRQM
Shipped at room temperature
Purity and Biological Activity of Human IL-6
| Fig. 1: SDS-PAGE analysis of recombinant human IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue. |
| Fig. 2: Measurement of the dose-dependent stimulation of cell proliferation in mouse hybridoma cell line B9 by IL-6. The cells were stimulated with increasing amounts of recombinant human IL-6. Values are the means (±SD) of triplicate determinations and expressed as percentage of control. |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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Payment Methods
Paying by bank transfer
