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Human Immunoglobulin G2 (IgG2-M)
Native, IgG subclass 2 whole molecule purified from myeloma plasma
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Proteins Synonyms: IgG2 Source: Human Plasma Purity: ≥ 95% by SDS-PAGE Physical State: Frozen in 20 mM phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3. Manufacturer: |
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Athens Research & Technology
Produktdetails
Synonyms
IgG2
Description of Human Immunoglobulin G2 (IgG2-M)
Human Immunoglobulin G2 (IgG2-M) is a 146 kDa protein. Serum IgG2 constitutes approximately 23% of total immunoglobulin G and is distinguished by a truncated 12-amino-acid hinge region with a poly-proline helix stabilized by four interchain disulfide bonds. This rigid structure limits Fab arm flexibility, reducing Fcγ receptor (FcγR) binding and complement activation compared to IgG1/IgG3. However, IgG2 excels in targeting repetitive carbohydrate antigens on encapsulated bacteria like Streptococcus pneumoniae and Haemophilus influenzae, forming high-avidity interactions critical for polysaccharide immunity. Its Fc domain binds FcγRIIA-H131 with moderate affinity, enabling phagocyte-mediated pathogen clearance in specific contexts. Deficiency in IgG2 (<2.1 g/L) impairs anti-polysaccharide responses, increasing susceptibility to recurrent sinopulmonary infections and invasive bacterial diseases. This deficiency often coexists with IgG4 or IgA deficits, complicating immune competence. Clinically, serum IgG2 quantification aids in evaluating humoral function, particularly in patients with chronic infections or hypogammaglobulinemia. Therapeutically, IgG2’s reduced Fc-mediated effector functions are exploited in oncology. Panitumumab, an IgG2 anti-EGFR monoclonal antibody, minimizes cytokine release syndrome while retaining tumor-targeting efficacy. Its inefficient FcRn-mediated transcytosis-attributed to the absence of Gly236 in the hinge-also results in lower placental transfer (~50% maternal levels), reducing fetal exposure risks. Emerging engineering strategies leverage IgG2’s structural stability for prolonged serum half-life and minimized off-target activation, enhancing therapeutic safety profiles.
Source
Human myeloma plasma non-reactive for HBsAG, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests
Storage
For long term, store Human Immunoglobulin G2 (IgG2-M) at ≤ -80°C.
Applications
Glycoproteomics, Inflammation, Biotherapeutics, In Vitro Diagnostic, Antimicrobial, Infection,.
Citations/Publications
Li, Y., et al., (2024), 'CD177 is a novel IgG Fc receptor and CD177 genetic variants affect IgG-mediated function', Front. Immunol. 15: 1418539. Available at: doi: 10.3389/fimmu.2024.1418539
Scarrone, M., et al., (2021), 'Development of anti-human IgM nanobodies as universal reagents for general immunodiagnostics', New BIOTECHNOLOGY 64: pp 9–16. Available at: https://doi.org/10.1016/j.nbt.2021.05.002
Hinneburg, H., et al., (2016), 'The Art of Destruction: Optimizing Collision Energies in Quadrupole-Time of Flight (Q-TOF) Instruments for Glycopeptide-Based Glycoproteomics', J. Am. Soc. Mass Spectrom. 201(27): pp 507-519. Available at: DOI: 10.1007/s13361-015-1308-6
Shipped with dry ice
Gel Scan of Human Immunoglobulin G2 (IgG2-M)
| Fig. 1: SDS-PAGE Gel |
Usage: For research use only. Not for use in diagnostic or therapeutic procedures. Not for human use.
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